Cancer
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Cancer
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Colon cancerMiR-155-5p controls colon cancer cell migration via post-transcriptional regulation of Human Antigen R (HuR).
Al-Haidari A1, Algaber A1, Madhi R1, Syk I1, Thorlacius H2.Author information Abstract Colorectal cancer (CRC) is the third most common cancer and a significant cause of cancer-related deaths worldwide. Metastasis is the worst prognostic factor for patients with CRC. HuR (ELAVL1) is overexpressed in CRC and has been reported to promote colon cancer growth by targeting RNA in the cell cytoplasm. Herein, the role of miR-155-5p in regulating HuR expression and cell migration was examined in colon cancer cells. MiR-155-5p knockdown in serum-starved colon cancer cells decreased both colon cancer cell chemotaxis and cytoplasmic expression of HuR. Bioinformatics analysis predicted two putative binding sites in the AU-rich elements (AREs) at the 3'-UTR of HuR mRNA. MiR-155-5p binding to HuR was verified using specific target site blockers and functionally validated by use of RNA immunoprecipitation assays, showing that miR-155-5p-dependent regulation of HuR expression is mediated by AREs. Targeting AREs with a specific blocker inhibited colon cancer cell migration. Taken together, these novel findings demonstrate that AREs mediate miR-155-5p positive regulation of HuR mRNA levels and translation as well as migration in colon cancer cells, suggesting that targeting miR-155-5p and/or Hur might be useful therapeutic strategies against colon cancer metastasis. KEYWORDS: Cell migration; Colon cancer; HuR; Metastasis; microRNA
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Tue Jun 23, 2020 7:21 pm by Raed Madhi
» Corona virus training course
Thu Apr 16, 2020 8:22 pm by Raed Madhi
» Platelet IP6K1 regulates neutrophil extracellular trap-microparticle complex formation in acute pancreatitis
Mon Oct 21, 2019 10:09 am by Raed Madhi
» SCI-HUB for not accessed articles.
Wed May 29, 2019 9:54 am by Raed Madhi
» Immunogenetic and prostate cancer
Thu Jan 17, 2019 9:22 pm by Bassam Ali
» Novel observation of NETs formation in AP by Meza et al 2015 ....
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